| Instrument | Conditions | ||
|---|---|---|---|
| Column | SunfireTM C18 5.0 μm, 4.6 mm×250 mm | ||
| Column temp. | 40℃ | ||
| Mobile phase (Gradient) | Time (min) | Solvent A1) | Solvent B2) |
| 0 | 8 | 92 | |
| 23 | 8 | 92 | |
| 26 | 15 | 85 | |
| 36 | 30 | 70 | |
| 40 | 45 | 55 | |
| 43 | 45 | 55 | |
| 45 | 8 | 92 | |
| 53 | 8 | 92 | |
| Detector | Waters 996 Photodiode Array Detector (280 nm) | ||
| Flow rate | 1.0 mL/min | ||
| Injection volume | 10 μL | ||
| Run time | 53 min | ||