Table 2. PCR conditions

		Denaturation	Annealing	Extension	Cycle
in vitro	GLUT4	94°C, 45 sec	57°C, 30 sec	72°C, 45 sec	34
	GAPDH	94°C, 45 sec	55°C, 30 sec	72°C, 45 sec	34
in vivo	GLUT4	94°C, 30 sec	66°C, 45 sec	72°C, 35 sec	28
	IST1	94°C, 30 sec	66°C, 45 sec	72°C, 35 sec	34
	Akt2	94°C, 30 sec	66°C, 45 sec	72°C, 35 sec	28
	β-actin	94°C, 30 sec	66°C, 45 sec	72°C, 35 sec	27