Table 3. Minimum bactericidal concentration (mg/mL) of pure isothiocyanates and radish root hydrolysate against fish pathogenic bacteria

Isothiocyanates		Fish pathogenic bacteria
		S. parauberis	S. iniae	E. tarda	V. ichthyoenteri	V. harveyi	P. damselae
		Aliphatic ITC1)
	Sulforaphane	0.875±0.250c3)	0.281±0.157bc	0.125±0.000c	>1.000	0.125±0.000b	0.250±0.000cd
	Sulforaphene	1.000±0.000c	1.000±0.000a	0.219±0.063c	>1.000	0.219±0.063b	0.250±0.000cd
	Iberin	1.000±0.000c	1.000±0.000a	0.500±0.000b	>1.000	0.250±0.000b	0.500±0.000b
	Erucin	0.750±0.289cd	0.500±0.354b	0.125±0.000c	>1.000	0.063±0.000b	0.125±0.000d
	Allyl ITC	4.000±0.000a	>4.000	0.500±0.000b	>4.000	1.313±1.796a	0.833±0.289a
	Hexyl ITC	>4.000	>4.000	>4.000	>4.000	>4.000	>4.000
Aromatic ITC
	Phenylethyl ITC	0.313±0.125cd	1.125±0.629a	0.438±0.125b	1.000±0.000a	0.125±0.000b	0.313±0.125bcd
	Benzyl ITC	0.625±0.250cd	0.250±0.000bc	0.500±.0.354b	1.000±0.000a	0.125±0.000b	0.438±0.125bc
	Phenyl ITC	2.000±1.414b	>4.000	>4.000	>4.000	>4.000	>4.000
Indolyl ITC
	Indole-3-carbinol	0.375±0.144cd	0.250±0.000bc	1.000±0.000a	0.813±0.800a	0.500±0.000ab	0.438±0.125bc
Hydrolysate of radish root		0.500±0.000cd	0.250±0.000bc	>0.500	>0.500	0.500±0.000ab	>0.500
Oxytetracycline2)		0.0008±0.0000d	0.0047±0.0018c	0.0031±0.000c	0.0027±0.0024b	0.0008±0.0000b	0.350±0.100bcd

ITC, isothiocyanate.
Oxytetracycline, positive control.
Values are expressed as mean±SD (n=4). Means with different superscripts in the same column are significantly different by Duncan’s multiple rage test (p<0.05).