| LC | Parameters | Conditions | ||
| Column | Phenomenex Phenylhexyl (100 2.1 mm, 3.5 μm) | |||
| Mobile phase | (A) 1% Acetic acid in water, (B) Methanol | |||
| A: 95% (0-2 min)→ 5% (2-10 min) → 95% (10-15 min) B: 5% (0-2 min)→ 95% (2-10 min) → 5% (10-15 min) | ||||
| Flow rate | 0.4 mL/min | |||
| Injection volume | 5 μL | |||
| Column temperature | 35°C | |||
| MS/MS | Mode | ESI (Electro-Spray Ionization), MRM (Positive) | ||
| Spray voltage | 4.0 kV | |||
| Sheath gas | 40 | |||
| Aux gas | 15 | |||
| Capillary temp. | 270°C | |||
| Collision pressure | 1.5 | |||
| Compounds | MRM transition | Tube lens (V) | Collision (V) | |
| T-2 toxin | 489.157→ 245.0 (Quantitation ion) 489.157→ 387.0 (Qualification ion) | 168 | 27 22 | |
| HT-2 toxin | 447.101→ 285.0 (Quantitation ion) 447.102→ 345.0 (Qualification ion) | 180 | 18 22 | |