Table 3.
Changes in free amino acid content of mash based on varying amount of water added
| Compounds | Free amino acid concentration (mg/100 mL) |
| 120% | 150% | 180% | 200% | 250% | 300% |
| Alanine | 52.74(11.1) | 33.44(10.5) | 24.38(10.1) | 24.20(9.9) | 19.62(10.4) | 24.33(11.7) |
| Ammonia | 2.93(0.6) | 2.23(0.7) | 2.00(0.8) | 1.99(0.8) | 1.74(0.9) | 1.60(0.8) |
| Anserine | n.d. | n.d. | n.d. | 0.85(0.3) | n.d. | n.d. |
| Arginine | 62.76(13.2) | 42.79(13.5) | 33.98(14.0) | 32.30(13.2) | 26.36(13.9) | 21.85(10.5) |
| Asparticacid | 18.90(4.0) | 9.09(2.9) | 4.81(2.0) | 5.49(2.2) | 3.63(1.9) | 4.93(2.4) |
| α-Aminoadioicacid | 2.44(0.5) | 2.25(0.7) | 2.14(0.9) | 1.94(0.8) | 0.89(0.5) | 1.17(0.6) |
| α-Aminobutyricacid | 0.24(0.1) | 0.22(0.1) | 1.64(0.7) | 0.44(0.2) | 0.73(0.4) | 0.18(0.1) |
| β-Alanine | 1.40(0.3) | 1.49(0.5) | 1.54(0.6) | 1.20(0.5) | 1.33(0.7) | 1.38(0.7) |
| β-Aminoisobutyricacid | 1.04(0.2) | 6.30(2.0) | 5.80(2.4) | 3.25(1.3) | 2.99(1.6) | 3.59(1.7) |
| γ-Aminobutyricacid | 6.16(1.3) | 5.74(1.8) | 2.50(1.0) | 3.98(1.6) | 2.09(1.1) | 3.61(1.7) |
| Carnosine | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Cystathionine | 5.74(1.2) | 4.75(1.5) | 4.08(1.7) | 4.38(1.8) | 3.61(1.9) | 3.89(1.9) |
| Cysteine | 9.08(1.9) | 8.56(2.7) | 7.20(3.0) | 3.63(1.5) | 2.63(1.4) | 1.83(0.9) |
| Ethanolamine | 0.37(0.1) | 0.26(0.1) | 0.22(0.1) | 0.24(0.1) | 0.12(0.1) | 0.17(0.1) |
| Glutamic acid | 41.93(8.8) | 26.01(8.2) | 19.31(8.0) | 21.19(8.7) | 15.01(7.9) | 20.94(10.1) |
| Glycine | 20.31(4.3) | 12.49(3.9) | 8.14(3.4) | 8.04(3.3) | 5.49(2.9) | 5.45(2.6) |
| Histidine | 7.58(1.6) | 6.11(1.9) | 4.91(2.0) | 4.71(1.9) | 4.12(2.2) | 3.67(1.8) |
| Hydroxylysine | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Hydroxyproline | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Isoleucine | 17.16(3.6) | 10.91(3.4) | 8.14(3.4) | 9.30(3.8) | 6.56(3.5) | 8.65(4.2) |
| Leucine | 35.84(7.6) | 21.81(6.9) | 15.57(6.4) | 17.96(7.3) | 12.51(6.6) | 15.68(7.5) |
| Lysine | 33.69(7.1) | 25.21(7.9) | 20.43(8.4) | 20.62(8.4) | 16.70(8.8) | 16.63(8.0) |
| Methionine | 9.18(1.9) | 7.21(2.3) | 5.72(2.4) | 6.08(2.5) | 4.88(2.6) | 4.34(2.1) |
| 1-Methylhistidine | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| 3-Methylhistidine | 1.24(0.3) | 0.48(0.1) | n.d. | n.d. | 0.28(0.1) | n.d. |
| Ornithine | 5.06(1.1) | 2.22(0.7) | 1.37(0.6) | 1.16(0.5) | 1.06(0.6) | 1.27(0.6) |
| Phenylalanine | 26.54(5.6) | 15.47(4.9) | 11.00(4.5) | 12.27(5.0) | 8.87(4.7) | 10.06(4.8) |
| Phosphoethanolamine | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Phosphoserine | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| Proline | 24.15(5.1) | 23.46(7.4) | 21.22(8.8) | 20.81(8.5) | 18.76(9.9) | 17.44(8.4) |
| Sarcosine | 0.84(0.2) | 1.60(0.5) | 1.65(0.7) | 1.59(0.6) | 1.12(0.6) | 0.93(0.4) |
| Serine | 22.99(4.8) | 12.51(3.9) | 7.10(2.9) | 8.27(3.4) | 4.82(2.6) | 6.93(3.3) |
| Taurine | 3.78(0.8) | n.d. | 3.14(1.3) | n.d. | 2.73(1.4) | 2.08(1.0) |
| Threonine | 13.01(2.7) | 6.84(2.2) | 4.05(1.7) | 5.07(2.1) | 3.02(1.6) | 4.91(2.4) |
| Tyrosine | 24.28(5.1) | 14.70(4.6) | 10.48(4.3) | 11.39(4.7) | 8.58(4.5) | 9.20(4.4) |
| Typtophan | n.d. | n.d. | n.d. | n.d. | n.d. | 0.45(0.2) |
| Urea | 0.36(0.1) | 0.41(0.1) | 0.48(0.2) | 0.33(0.1) | 0.44(0.2) | 0.24(0.1) |
| Valine | 22.86(4.8) | 12.75(4.0) | 8.91(3.7) | 11.86(4.8) | 8.31(4.4) | 10.70(5.1) |
|
| Total | 474.60 | 317.32 | 241.89 | 244.51 | 189.00 | 208.12 |
n.d. means not detected.
The numbers in parentheses indicate the percentage distribution of each compound.