Condition | |
---|---|
Column | Shim-pack ISA-07/S2504(250 4.0 mm) |
Mobile phase | A: Potassium borate(pH 8) |
B: Potassium borate(pH 9) | |
Flow rate | 0.5 mL/min[0→15 min: B(0 → 20%), 30 min: B(20 → 50%), 45 min: B(50 → 90%), 50 min: B(90 → 100%), 65 min: B(100 → 100%), 90 min: B(100 → 0%)] |
Oven temperature | 65°C |
Reagent | 1% arginine in 3% boric acid |
Reagent temperature | 150°C |
Detector | Fluorescence detector Ex=320 nm, Em=430 nm |