| Instrument | Shiseido HPLC system | ||
|---|---|---|---|
| Detector | Shiseido ACCELA photo diode array | ||
| Wavelength | 217 nm | ||
| Column | hiseido CAPCELL PAK C8 SG80 5 um 4.6 mm I.D. 150 mm | ||
| Mobile phase | A solution : 0.1% TBA-OH (0.1% Phosphoric acid) solution | ||
| B solution : Acetonitrile | |||
| Gradient condition | Time (min) | A (%) | B (%) |
| 0.0 | 75 | 25 | |
| 2.5 | 75 | 25 | |
| 7.0 | 65 | 35 | |
| 12.0 | 60 | 40 | |
| 15.0 | 70 | 30 | |
| Flow rate | 1.0 mL/min | ||
| Injection volume | 10 uL | ||