Table 2.
Enzyme activities of bacter ia separated from traditional meju and koji
Bacteria No. | α-Amylase (unit/g)3) | β-Amylase (unit/g)4) | Acidic protease (unit/g)5) | Neutral protease (unit/g)6) |
1-B-11) | 572.44 | 2140.38 | 391.76 | 316.46 |
2-B-11) | 1487.00 | 1979.85 | 309.15 | 277.62 |
3-B-11) | 1646.18 | 3953.13 | 877.56 | 790.08 |
4-B-11) | 1030.96 | 480.53 | 477.60 | 391.36 |
5-B-11) | 22.80 | 279.87 | 63.09 | 79.54 |
6-B-11) | 10< | 1784.98 | 109.98 | 46.56 |
7-B-11) | 153.57 | 1039.01 | 255.86 | 200.65 |
8-B-11) | 143.56 | 213.99 | 91.61 | 162.10 |
9-B-11) | 207.51 | 273.64 | 342.54 | 304.25 |
10-B-11) | 884.67 | 2700.44 | 956.55 | 1147.70 |
11-B-11) | 10< | 263.24 | 36.24 | 58.42 |
12-B-11) | 322.61 | 3097.06 | 1083.38 | 1197.87 |
13-B-11) | 842.74 | 574.84 | 103.04 | 94.96 |
14-B-11) | 607.57 | 761.57 | 147.56 | 124.13 |
15-B-11) | 871.86 | 914.53 | 306.32 | 266.96 |
16-B-11) | 170.49 | 283.31 | 84.24 | 108.42 |
17-B-11) | 10< | 519.57 | 923.42 | 772.09 |
18-B-11) | 10< | 298.00 | 381.38 | 321.77 |
19-B-11) | 643.41 | 2321.69 | 255.57 | 591.47 |
20-B-11) | 10< | 343.21 | 10< | 58.98 |
21-B-11) | 49.61 | 339.28 | 10< | 70.57 |
22-B-11) | 85.60 | 378.28 | 72.80 | 86.99 |
23-B-11) | 117.93 | 185.30 | 130.28 | 256.24 |
24-B-11) | 106.75 | 160.60 | 114.78 | 208.27 |
25-B-11) | 348.75 | 1361.44 | 328.20 | 425.34 |
26-B-11) | 1073.66 | 2811.27 | 496.08 | 705.44 |
27-B-11) | 1426.76 | 3676.80 | 701.19 | 1012.74 |
28-B-11) | 464.53 | 1051.49 | 508.43 | 784.85 |
29-B-11) | 44.53 | 237.76 | 479.10 | 594.50 |
30-B-11) | 382.36 | 330.20 | 66.50 | 126.00 |
31-B-11) | 868.31 | 2088.24 | 571.49 | 887.77 |
32-B-11) | 63.57 | 210.21 | 62.24 | 112.56 |
33-B-11) | 56.69 | 211.93 | 72.80 | 69.91 |
34-B-11) | 144.49 | 242.55 | 84.81 | 134.84 |
35-B-11) | 57.53 | 153.48 | 27.37 | 70.61 |
36-B-11) | 398.72 | 1169.97 | 377.76 | 259.14 |
37-B-11) | 157.30 | 1318.95 | 217.28 | 131.68 |
38-B-11) | 1008.00 | 3877.63 | 322.35 | 322.66 |
39-B-11) | 172.00 | 162.41 | 43.16 | 10< |
40-B-11) | 426.38 | 2507.49 | 483.78 | 667.82 |
41-B-11) | 68.37 | 223.39 | 281.90 | 469.62 |
1-K-B-12) | 398.72 | 1169.97 | 377.76 | 259.14 |
2-K-B-12) | 157.30 | 1318.95 | 217.28 | 131.68 |
3-K-B-12) | 1008.00 | 3877.63 | 322.35 | 322.66 |
4-K-B-12) | 1226.19 | 3173.98 | 433.34 | 593.02 |
5-K-B-12) | 521.50 | 1315.60 | 492.48 | 271.18 |
Bacteria separated from traditional Meju.
Bacteria separated from Koji.
One unit of α-amylase was the amount of enzyme which released reducing sugar from 1 mg soluble starch for 10 minutes at 25°C and pH 5.9 under specific conditions.
One unit of β-amylase was the amount of enzyme which released maltose from 1 mg soluble starch for 10 minutes at 25°C and pH 5.9 under specific conditions.
One unit of acidic protease was the amount of enzyme which released 1 µg tyrosine from 1 mg casein for 10 minutes at 30°C and pH 3.0 under specific conditions.
One unit of neutral protease was the amount of enzyme which released I µg tyrosine from 1 mg casein for 10 minutes at 30°C and pH 6.0 under specific conditions.