Table 1. Conditions for HPLC analysis of 60% ethanol extracts from T. radicans and fermented T. radicans

Instrument	HP HPLC series 1100 (Hewlett Packard, Waldbronn, Germany)
Column	ZORBAX RX-SIL (4.6 mm×250 mm, 5 μm) column
Column temp	23°C
Injection volume	10 μL
Mobile phase	Phase A: 100% methanol,Phase B: distilled water,Phase C: 100% ACN (A:B:C=20:60:20)
Flow rate	0.7 mL/min
Retention time	30 min
Detection	UV absorption (230 nm)