Table 1. LC-MS/MS conditions for the analysis of vitamin D

HPLC (Ekspert ultraLC 110-XL) conditions
Column	CAPCELL CORE C18 (90 Ǻ×2.7 μm×2.1 mm×150 mm)
Mobile phase	5 mM ammonium acetate:methanol (5:95)
Oven temperature	60°C
Inject volume	10 μL
MS/MS (Triple Quad 4500) conditions
Ionization	ESI positive
Source temperature	700°C
Collision gas	N2
	Vitamin D2	Vitamin D3
Precursor ion (m/z)	397	385
Fragment ion (m/z)	107, 105, 91	107, 105, 79