Table 3. Monomer and dimer tannin concentration of grape seed at different solvents condition by HPLC-DAD
Solvents | Seed (mg/kg) |
catechin | epicatechin | gallocatechin | epigallocatechin | procyanidin B1 | procyanidin B2 | total |
Methanol | 100% | 535.6±66.64ab,1)2) | 143.5±9.59bc | 115.6±28.80ab | 421.3±59.05cd | 1,336.8±87.87b | 202.8±145.90a | 2,755.6±81.91b |
70% | 774.9±144.30a | 209.4±22.51c | 141.8±20.90a | 598.3±46.69b | 1,741.3±132.93a | 117.9±8.73b | 3,583.5±372.73a |
70%+0.5% HCl | 40.3±8.89d | 44.0±6.10d | 12.2±4.33c | 3.0±5.94g | 72.8±24.16f | 12.6±9.79c | 184.8±22.27d |
Ethanol | 100% | 453.2±22.99c | 129.1±12.36c | 16.2±32.32c | 277.6±19.13de | 1,052.0±50.43c | 56.1±3.07bc | 1,984.3±134.97b |
70% | 506.1±27.90bc | 155.7±7.41bc | 122.1±8.63ab | 461.6±21.64bc | 1,312.5±52.06b | 89.2±3.07bc | 2,647.2±134.97b |
70%+0.5% HCl | 29.9±2.66d | 28.5±19.08d | 47.4±6.32c | 168.3±40.35ef | 178.6±13.33f | 5.7±11.33c | 458.4±72.24cd |
Acetone | 100% | 469.5±24.44c | 174.7±20.82ab | 99.9±16.98b | 0.0±0.00g | 435.8±145.34e | 58.7±32.63bc | 1,238.6±196.77c |
70% | 692.7±207.65ab | 200.5±24.18a | 143.0±19.39a | 1,940.0±150.52a | 812.6±147.12d | 119.9±10.22b | 3,908.7±547.98a |
70%+0.5% HCl | 23.0±2.97d | 30.4±2.79d | 40.2±5.33c | 86.5±15.06fg | 130.1±2.74f | 8.2±0.14c | 318.4±25.59d |
1)Mean±Standard deviation. (n=4)
2)Different letters are significantly different at p<0.05 by Duncan’s multiple range test (DMRT).
3)N.D = Not detected.