Table 3. Monomer and dimer tannin concentration of grape seed at different solvents condition by HPLC-DAD

Solvents Seed (mg/kg)
catechin epicatechin gallocatechin epigallocatechin procyanidin B1 procyanidin B2 total
Methanol 100% 535.6±66.64ab,1)2) 143.5±9.59bc 115.6±28.80ab 421.3±59.05cd 1,336.8±87.87b 202.8±145.90a 2,755.6±81.91b
70% 774.9±144.30a 209.4±22.51c 141.8±20.90a 598.3±46.69b 1,741.3±132.93a 117.9±8.73b 3,583.5±372.73a
70%+0.5% HCl 40.3±8.89d 44.0±6.10d 12.2±4.33c 3.0±5.94g 72.8±24.16f 12.6±9.79c 184.8±22.27d
Ethanol 100% 453.2±22.99c 129.1±12.36c 16.2±32.32c 277.6±19.13de 1,052.0±50.43c 56.1±3.07bc 1,984.3±134.97b
70% 506.1±27.90bc 155.7±7.41bc 122.1±8.63ab 461.6±21.64bc 1,312.5±52.06b 89.2±3.07bc 2,647.2±134.97b
70%+0.5% HCl 29.9±2.66d 28.5±19.08d 47.4±6.32c 168.3±40.35ef 178.6±13.33f 5.7±11.33c 458.4±72.24cd
Acetone 100% 469.5±24.44c 174.7±20.82ab 99.9±16.98b 0.0±0.00g 435.8±145.34e 58.7±32.63bc 1,238.6±196.77c
70% 692.7±207.65ab 200.5±24.18a 143.0±19.39a 1,940.0±150.52a 812.6±147.12d 119.9±10.22b 3,908.7±547.98a
70%+0.5% HCl 23.0±2.97d 30.4±2.79d 40.2±5.33c 86.5±15.06fg 130.1±2.74f 8.2±0.14c 318.4±25.59d
1)Mean±Standard deviation. (n=4)
2)Different letters are significantly different at p<0.05 by Duncan’s multiple range test (DMRT).
3)N.D = Not detected.