Table 5. The contents of free amino acid in Tenebrio molitor L. ingested yuzu supplemented feed

(mg%)

Sample¹⁾ C120 C80+Y40 C40+Y80 Y120

Essential amino acids Valine 207.67±34.01^NS2) 245.83±2.46 209.43±38.52 208.62±53.78

Leucine 96.84±27.72^bc3) 133.21±24.91^a 120.16±35.51^ab 79.02±16.94^c

Isoleucine 102.22±45.38^NS 117.62±48.59 112.05±44.28 113.26±45.98

Lysine 185.64±9.45^NS 170.28±39.77 197.81±25.89 156.70±10.56

Histidine 233.27±28.90^a 195.02±39.26^ab 195.18±64.12^ab 171.66±36.00^b

Methionine 44.88±3.67^NS 42.64±14.15 48.59±33.00 38.53±17.87

Threonine 44.21±55.19^NS 92.88±11.27 43.58±40.47 83.68±13.54

Phenylalanine 52.88±9.73^ab 80.82±25.75^a 72.58±19.71^ab 37.31±20.76^b

Non-essential amino acids Proline 477.48±76.06^NS 515.95±39.87 475.00±22.40 470.08±92.81

Alanine 292.59±83.52^NS 350.22±44.43 333.85±11.22 331.73±66.76

Tyrosine 182.72±18.43^NS 246.42±60.45 236.08±72.60 229.50±54.17

Arginine 380.41±49.46^NS 351.33±11.31 337.05±61.94 335.15±47.42

Glutamic acid 134.80±39.79^NS 106.42±68.28 127.72±97.09 144.21±32.49

Glycine 80.25±15.24^NS 84.04±15.20 81.55±15.09 65.29±5.25

Serine 22.27±13.32^NS 34.05±7.84 30.95±2.43 29.93±6.82

Aspartic acid 18.14±13.32^NS 18.81±4.83 12.81±3.29 18.44±11.74

Asparagine 91.89±72.96^NS 57.97±46.72 68.94±38.90 154.12±186.51

Cystine 16.64±4.77^NS 23.97±16.90 24.83±4.38 14.93±3.71

EAA⁴⁾ 967.61 1,078.30 999.38 888.78

TAA 2,784.8 2,947.48 2,768.16 2,802.16

¹⁾See the legend of Table 1.
²⁾NS, not significant.
³⁾Mean±SD with different superscripts within the same column are significantly different (p<0.05) by Duncan’s multiple range test. a>b>c.
⁴⁾EAA: essential amino acids; TAA: total amino acids.

Sample¹⁾		C120	C80+Y40	C40+Y80	Y120
Essential amino acids	Valine	207.67±34.01^NS2)	245.83±2.46	209.43±38.52	208.62±53.78
	Leucine	96.84±27.72^bc3)	133.21±24.91^a	120.16±35.51^ab	79.02±16.94^c
	Isoleucine	102.22±45.38^NS	117.62±48.59	112.05±44.28	113.26±45.98
	Lysine	185.64±9.45^NS	170.28±39.77	197.81±25.89	156.70±10.56
	Histidine	233.27±28.90^a	195.02±39.26^ab	195.18±64.12^ab	171.66±36.00^b
	Methionine	44.88±3.67^NS	42.64±14.15	48.59±33.00	38.53±17.87
	Threonine	44.21±55.19^NS	92.88±11.27	43.58±40.47	83.68±13.54
	Phenylalanine	52.88±9.73^ab	80.82±25.75^a	72.58±19.71^ab	37.31±20.76^b
Non-essential amino acids	Proline	477.48±76.06^NS	515.95±39.87	475.00±22.40	470.08±92.81
	Alanine	292.59±83.52^NS	350.22±44.43	333.85±11.22	331.73±66.76
	Tyrosine	182.72±18.43^NS	246.42±60.45	236.08±72.60	229.50±54.17
	Arginine	380.41±49.46^NS	351.33±11.31	337.05±61.94	335.15±47.42
	Glutamic acid	134.80±39.79^NS	106.42±68.28	127.72±97.09	144.21±32.49
	Glycine	80.25±15.24^NS	84.04±15.20	81.55±15.09	65.29±5.25
	Serine	22.27±13.32^NS	34.05±7.84	30.95±2.43	29.93±6.82
	Aspartic acid	18.14±13.32^NS	18.81±4.83	12.81±3.29	18.44±11.74
	Asparagine	91.89±72.96^NS	57.97±46.72	68.94±38.90	154.12±186.51
	Cystine	16.64±4.77^NS	23.97±16.90	24.83±4.38	14.93±3.71
EAA⁴⁾		967.61	1,078.30	999.38	888.78
TAA		2,784.8	2,947.48	2,768.16	2,802.16