Table 1. The chemical properties of crude and fractionated polysaccharides isolated from Styela plicata
Sample1) | Yield(dry basis, %) | Total carbohydrate (glucose, %) | Uronic acid(GA2),%) | Sulfate (%) | Protein(BSA3),%) | Molecular weight(kDa) |
SP | 11.90±0.51b4) | 28.60±0.88c | 9.88±0.11d | 18.03±0.41d | 26.47±0.22a | - |
SF-1 | 7.02±0.09c | 42.65±1.08a | 23.76±0.89a | 29.25±0.27a | 1.72±0.15c | 1,187 |
SF-2 | 24.18±0.68a | 33.36±0.64b | 17.01±1.23c | 23.22±0.22c | 9.23±0.32b | 136 |
SF-3 | 10.80±0.24b | 28.03±1.08c | 20.90±0.56b | 27.09±0.34b | 1.48±0.18c | 28 |
1)SP, crude polysaccharide from Styela plicata; SF-1, polysaccharide fraction-Ⅰ; SF-2, polysaccharide fraction-Ⅱ; SF-3, polysaccharide fraction-Ⅲ.
2)Uronic acid content was determined by the carbazole reaction, using galacturonic acid (GA) as a standard.
3)BSA, bovine serum albumin.
4)Means±SD (n=3) within each column (a-d) followed by the same letter are not significantly different (p<0.05).