Table 1. The chemical properties of crude and fractionated polysaccharides isolated from Styela plicata

Sample1) Yield(dry basis, %) Total carbohydrate (glucose, %) Uronic acid(GA2),%) Sulfate (%) Protein(BSA3),%) Molecular weight(kDa)
SP 11.90±0.51b4) 28.60±0.88c 9.88±0.11d 18.03±0.41d 26.47±0.22a -
SF-1 7.02±0.09c 42.65±1.08a 23.76±0.89a 29.25±0.27a 1.72±0.15c 1,187
SF-2 24.18±0.68a 33.36±0.64b 17.01±1.23c 23.22±0.22c 9.23±0.32b 136
SF-3 10.80±0.24b 28.03±1.08c 20.90±0.56b 27.09±0.34b 1.48±0.18c 28
1)SP, crude polysaccharide from Styela plicata; SF-1, polysaccharide fraction-Ⅰ; SF-2, polysaccharide fraction-Ⅱ; SF-3, polysaccharide fraction-Ⅲ.
2)Uronic acid content was determined by the carbazole reaction, using galacturonic acid (GA) as a standard.
3)BSA, bovine serum albumin.
4)Means±SD (n=3) within each column (a-d) followed by the same letter are not significantly different (p<0.05).