Table 3. Identification of false positive colony on Sanita-kun coliform count

Biochemical test Colony number
R4-S-6 P13-S-2 P20-S-4 G7-S-8 G30-S-7 S22-S-4
Lactose broth Gas (-) Gas (-) Gas (-) Gas (-) Gas (-) Gas (-)
Gram stain Positive Positive Positive Positive Positive Positive
Spore stain + + + + + +
Catalase + + + + + +
Oxidase + + + + + +
Motility + + + + + +
ONPG + + + + + +
PCC1) Non-growth Non-growth Non-growth Non-growth Non-growth Non-growth
SCC2) Blue colony Blue colony Blue colony Blue colony Blue colony Blue colony
Vitek2 BCL Bacillus licheniformis - - - - Bacillus licheniformis
16S-rRNA sequencing Bacillus rhizosphaeraeorBacillus clausii Bacillus pumilusorBacillus safensis Bacillus pumilusorBacillus safensis Bacillus pumilusorBacillus safensis
1)PCC: Petrifilm coliform count (incubation at 35℃ for 24 h)
2)SCC: Sanita-kun coliform count (incubation at 35℃ for 24 h)