Table 2. Identification of false negative colony on Petrifilm coliform count

Biochemical test Colony number
P20-3M-N1 P20-3M-N5 P22-3M-N3 P22-3M-N4 G13-3M-N4 G16-3M-N1 G16-3M-N4 G30-3M-N4 G30-3M-N5
Lactose broth Gas (+) Gas (+) Gas (+) Gas (+) Gas (+) Gas (+) Gas (+) Gas (+) Gas (+)
Gram stain negative negative negative negative negative negative negative negative negative
Catalase + + + + + + + + +
Oxidase - - - - - - - - -
Motility + + + + + + + + +
ONPG + + + + + + + + +
PCC1) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-) Red colony, gas(-)
SCC2) Blue colony Blue colony Blue colony Blue colony Blue colony Blue colony Blue colony Blue colony Blue colony
API 20E Escherichia hermannii Escherichia hermannii Pantoea spp. 2 Pantoea spp. 3 Pantoea spp. 3 Pantoea spp. 2 Pantoea spp. 1 Pantoea spp. 2 Pantoea spp. 2
1)PCC: Petrifilm coliform count (incubation at 35℃ for 24 h)
2)SCC: Sanita-kun coliform count (incubation at 35℃ for 24 h)