| Condition | 1st. hydrolysis | 2nd. hydrolysis | extraction | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| protease (g) | trypsin (g) | time (h) | pH | bromelain (g) | papain (g) | time (h) | pH | vacuum (torr) | time (h) | |||
| G | 1 | 1 | 4 | - | 1 | 1 | 16 | - | - | 0 | ||
| H | 1 | 1 | 4 | - | 1 | 1 | 16 | - | - | 4 | ||
| I | 1 | 1 | 4 | 7.5 | 1 | 1 | 16 | 5.5 | - | 0 | ||
| J | 1 | 1 | 4 | 7.5 | 1 | 1 | 16 | 5.5 | - | 4 | ||
| K | 1 | 1 | 8 | - | 1 | 1 | 16 | - | - | 4 | ||
| L | 1 | 1 | 8 | - | 1 | 1 | 16 | - | 300 | 4 | ||