Table 2. HPLC conditions for analysis of DNJ in mulberry leaf and tea

Item	Operation condition
HPLC system	Waters e2690/5 HPLC system equipped with 2998 photodiode array detector and autosampler
Column	YMC-Pack Pro C18(5 μm, 4.6×250 mm, YMC Inc., Allentown, PA, USA)
Flow rate	1.0 mL/min
Detector	UV254 nm
Gradient elution
		Time (min)	H2O	100% MeOH
		0	45	55
		16	45	55
		19	10	90
		22	10	90
		25	45	55
		30	45	55