Table 1. HPLC conditions for analysis of phenolic compounds in mulberry leaf and tea

Item	Operation condition
HPLC system	Waters e2690/5 HPLC system equipped with 2998 photodiode array detector and autosampler
Column	YMC-Pack Pro C18(5 μm, 4.6×250 mm, YMC Inc., Allentown, PA, USA)
Flow rate	0.8 mL/min
Detector	UV330, 350 nm
Gradient elution
		Time (min)	Acetonitrile	0.05% H3PO4 in H2O
		0	10	90
		20	20	80
		25	20	80
		40	30	70
		45	30	70
		50	90	10
		55	90	10
		60	10	90