Table 1. HPLC condition for the analysis of 4-Ο-caffeoylquinic acid
Item
Condition
Instrument
Waters Alliance e2695
(Waters Co.)
Detector
Waters 2489 UV detector 280 nm
(Waters Co.)
Column
Capcell pac C
18
column
(Shiseido Co., 250 mm L×4.6 mm I.D)
Solvent
0.05% TFA (A), Acetonitrile (B)
Time
A
B
0
99
1
10
92
8
20
92
8
25
88
12
35
88
12
40
84
16
50
84
16
55
80
20
65
80
20
Column temp.
40℃
Flow rate
1.0 mL/min
Injection volume
10 μL