Table 1. Hydrolysis conditions of proteolytic enzymes for perilla seed meal protein hydrolysis

Proteases Hydrolysis conditions
Temperature (℃) pH Buffer solution
Pepsin 37 2 0.1 M glycine-HCl buffer
Trypsin 37 8 0.1 M sodium phosphate buffer
Papain 37 6 0.1 M sodium phosphate buffer
Acalase 50 7 0.1 M sodium phosphate buffer
Protamex 50 7 0.1 M sodium phosphate buffer
Neutrase 50 7 0.1 M sodium phosphate buffer
Flavourzyme 50 7 0.1 M sodium phosphate buffer