Table 1. Proportion of fermented Cheonggukjang with different sugar contents

Groups1) Sterilization2) Sucrose (%) Starter3) (mL)
Con No - -
WS No - 0.5
WSS Yes - 0.5
Con (2.5S) No 2.5 -
WS (2.5S) No 2.5 0.5
WSS (2.5S) Yes 2.5 0.5
Con (7.5S) No 7.5 -
WS (7.5S) No 7.5 0.5
WSS (7.5S) Yes 7.5 0.5
1) Con, prior to 2nd fermentation, either 0, 2.5, 7.5% of sucrose was supplemented to fermentation broth then 2nd fermentation was carried out at 40℃ for 48 h; WS, to fermentation broth containing 40 g of Cheonggukjang and 2% lactic acid, sucrose solution was supplemented as indicated in Table 1 and 2nd fermentation was carried out at 40℃ for 48 h using L. acidophilus KCTC 3925; WSS, to fermentation broth, sucrose solution was supplemented and then heat-sterilized at 100℃, 15 min. 2nd fermentation was carried out at 40℃ for 48 h using L. acidophilus KCTC3925.
2) Sterilization at 100℃ for 15 min.
3) Freshly prepared L. acidophillus KCTC 3925 cultures after grown in MRS at 37℃ for 24 h, were used.