Table 3 Accuracy of immunoaffinity-HPLC/DAD assay for vitamin B12
Sample | Vitamin B12 content (μg/100 g) | Recovery3) (%) |
Reference value1) | Analysis value2) |
BCR® 487 | 112.00±9.00 | 111.51±11.51 | 99.6 |
SRM® 1546a | 0.55±0.16 | 0.52±0.09 | 95.0 |
Reference value indicates the vitamin B12 contents of BCR® 487 and SRM® 1546a provided by EC and NIST, respectively, as the certificate value.
Analysis value indicates the vitamin B12 contents obtained by immunoaffinity- HPLC/DAD assay for vitamin B12 in this study.
Recovery (%) was calculated by 100 (analytical value/reference value).