Table 3 Accuracy of immunoaffinity-HPLC/DAD assay for vitamin B12

Sample Vitamin B12 content (μg/100 g) Recovery3) (%)
Reference value1) Analysis value2)
BCR® 487 112.00±9.00 111.51±11.51 99.6
SRM® 1546a 0.55±0.16 0.52±0.09 95.0
Reference value indicates the vitamin B12 contents of BCR® 487 and SRM® 1546a provided by EC and NIST, respectively, as the certificate value.
Analysis value indicates the vitamin B12 contents obtained by immunoaffinity- HPLC/DAD assay for vitamin B12 in this study.
Recovery (%) was calculated by 100 (analytical value/reference value).