Table 2 Free amino acid composition of naked oat flours according to different particle sizes

Components1)		Control	Fractions of control
			Fraction A (>250 μm)	Fraction B (160-250 μm)	Fraction C (<160 μm)
			EAA (mg%)	Threonine	4.2±0.6^a2)	3.5±0.0^b	4.2±0.6^a	3.5±0.0^b
Valine	0.3±0.3^a	ND3)		0.5±0.0^a	0.5±0.0^a
Methionine	3.8±0.3^ab	4.0±0.0^a		3.1±0.8^b	1.8±0.3^c
Isoleucine	2.0±0.5^b	1.5±0.0^c		3.8±2.8^b	6.8±0.3^a
Leucine	4.0±0.5^b	3.5±0.0^c		5.3±2.0^b	7.0±0.5^a
Phenylalanine	8.6±1.9^ab	10.8±0.3^a		8.0±0.9^b	8.8±0.3^b
Histidine	12.2±3.6^a	8.0±0.0^b		11.6±4.4^a	6.3±0.3^c
Tryptophan	0.5±0.0^a	0.5±0.0^a		0.5±0.0^a	0.5±0.0^a
Lysine	3.5±0.0^b	3.5±0.0^a		3.5±0.0^a	3.5±0.0^a
Total	39.1±3.5^a	35.3±0.3^b		40.3±0.6^a	38.5±1.5^a
NEAA (mg%)	Phophoserine	3.7±0.3^a	4.0±0.0^a	3.8±0.6^a	4.3±0.3^a
	Phosphoethanol	ND	ND	ND	ND
	Taurine	ND	ND	ND	ND
	Aspartic acid	34.0±1.3^a	21.0±0.5^b	34.2±1.0^a	21.5±0.0^b
	Serine	10.6±4.6^ab	5.3±0.3^c	11.9±2.5^a	8.8±0.3^b
	Asparagine	5.0±1.8^a	3.0±0.0^b	4.3±2.5^ab	1.5±0.0^c
	Glutamic acid	53.8±7.0^ab	46.3±0.3^b	56.7±1.4^a	57.0±0.5^a
	α-Aminoadipic acid	ND	ND	12.8±2.2^b	38.0±0.5^a
	Glycine	6.7±1.4^ab	5.0±0.0^c	6.8±1.0^a	5.3±0.3^b
	Alanine	38.8±7.2^a	19.0±0.5^b	38.3±8.0^a	17.3±0.3^c
	Citrulline	3.8±3.3^a	ND	3.8±3.3^a	ND
	α-Aminobutyric acid	25.5±3.0^a	22.0±0.5^a	18.3±5.4^a	0.8±0.3^b
	Cystine	58.3±0.4^a	58.8±0.3^a	49.7±4.4^b	32.8±0.3^c
	Tyrosine	5.4±0.6^b	7.3±0.3^a	3.8±0.2^c	2.5±0.0^d
	β-Alanine	6.3±0.5^b	15.0±0.0^a	1.3±0.2^c	ND
	β-Aminoisobutyric acid	53.8±6.3^b	118.8±0.3^a	20.1±2.2^c	17.3±0.3^d
	γ-Aminobutyric acid	27.8±1.0^b	29.0±0.0^a	18.9±4.2^c	2.5±0.0^d
	Carnosine	4.3±0.9^d	10.0±0.0^c	19.0±0.3^b	53.8±0.3^a
	Ornithine	0.5±0.0^b	0.5±0.0^b	0.7±0.3^ab	1.0±0.0^a
	Ammonia	3.7±1.9^a	1.5±0.0^c	3.9±1.7^a	2.0±0.0^b
	Total	341.9±11.3^b	366.3±2.3^a	308.3±4.5^c	266.0±2.5^d
Total free amino acid content		381.0±18.3^b	401.5±2.5^a	348.6±34.8^c	304.5±4.0^d

EAA and NEAA are an abbreviation for essential amino acid and non essential amino acid, respectively.
Values are mean±SD (n=3). Means with different superscript small letters in the same row are significantly different by Duncan’s multiple test (p<0.05).
ND is an abbreviation for not detected.