Table 2 Central composite design matrix for total polyphenol content (TPC), total flavonoid content (TFC), and DPPH radical scavenging activity of Hordeum vulgare L. under microwave-assisted extraction conditions
No | Ethanol concentration (%) | Microwave power (W) | Extraction time (min) | TPC (mg GAE/g) | TFC (mg RE/g) | DPPH (%) |
1 | 25 | 120 | 8 | 3.17±0.081) | 2.50±0.15 | 30.14±0.23 |
2 | 75 | 120 | 8 | 3.60±0.09 | 4.58±0.08 | 34.67±0.29 |
3 | 25 | 120 | 16 | 3.55±0.08 | 2.89±0.22 | 30.90±0.48 |
4 | 75 | 120 | 16 | 3.87±0.05 | 5.74±0.37 | 37.21±0.41 |
5 | 25 | 240 | 8 | 3.45±0.04 | 2.35±0.00 | 29.95±0.24 |
6 | 75 | 240 | 8 | 3.63±0.04 | 4.87±0.08 | 35.92±0.48 |
7 | 25 | 240 | 16 | 3.64±0.02 | 2.40±0.08 | 30.02±0.50 |
8 | 75 | 240 | 16 | 3.88±0.05 | 9.04±0.15 | 38.05±0.35 |
9 | 0 | 180 | 12 | 2.64±0.03 | 0.80±0.08 | 21.58±0.41 |
10 | 100 | 180 | 12 | 3.3±0.03 | 9.33±0.15 | 34.44±0.59 |
11 | 50 | 180 | 4 | 3.05±0.07 | 2.84±0.08 | 30.86±0.40 |
12 | 50 | 180 | 20 | 3.89±0.05 | 4.82±0.29 | 41.86±1.47 |
13 | 50 | 60 | 12 | 3.34±0.07 | 2.69±0.08 | 27.05±0.41 |
14 | 50 | 300 | 12 | 3.79±0.11 | 4.53±0.29 | 30.71±0.57 |
15 | 50 | 180 | 12 | 3.68±0.10 | 3.76±0.17 | 33.11±0.34 |
16 | 50 | 180 | 12 | 3.67±0.05 | 3.72±0.17 | 33.37±0.48 |
Mean±SD (n=3).