Table 3 ABTS+ radical scavenging activity of 80% methanol extract of Mongolian I. bungei and its n-hexane, EtOAc-, n-BuOH-, and H2O-soluble fractions

Samples Inhibition (%) IC50 (μg/mL)
100 50 25 12.5 6.3
80% MeOH ext. 66.3±2.1d1) 49.7±1.3c 35.3±2.1c 18.8±2.1bc 9.8±1.3b 50.9±1.9
n-Hexane layer 48.4±1.4e 31.9±1.1d 13.9±0.1d 7.6±0.2cd 1.5±0.1c >200
EtOAc layer 89.2±1.8b 72.8±3.1b 43.6±1.3b 24.7±0.3b 10.0±0.8b 27.8±0.9
n-BuOH layer 40.1±1.3f 23.7±1.1d 12.6±1.0d 3.4±1.2d 0.9±1.4c >200
H2O layer 72.6±2.1c 51.6±2.3c 37.8±0.5c 15.2±1.4b 7.1±1.1b 45.2±3.0
(+)-Catechin2) 99.7±0.1a 97.5±0.1a 85.2±1.2a 39.3±1.1a 32.7±0.7a 14.6±0.4
Scavenging activities are expressed as the mean±SD of triplicate experiments. Mean separation within columns of respective seasons by Duncan’s multiple range test at p=0.05.
(+)-Catechin was used as a positive control.