Table 3 ABTS+ radical scavenging activity of 80% methanol extract of Mongolian I. bungei and its n-hexane, EtOAc-, n-BuOH-, and H2O-soluble fractions
Samples | Inhibition (%) | IC50 (μg/mL) |
100 | 50 | 25 | 12.5 | 6.3 |
80% MeOH ext. | 66.3±2.1d1) | 49.7±1.3c | 35.3±2.1c | 18.8±2.1bc | 9.8±1.3b | 50.9±1.9 |
n-Hexane layer | 48.4±1.4e | 31.9±1.1d | 13.9±0.1d | 7.6±0.2cd | 1.5±0.1c | >200 |
EtOAc layer | 89.2±1.8b | 72.8±3.1b | 43.6±1.3b | 24.7±0.3b | 10.0±0.8b | 27.8±0.9 |
n-BuOH layer | 40.1±1.3f | 23.7±1.1d | 12.6±1.0d | 3.4±1.2d | 0.9±1.4c | >200 |
H2O layer | 72.6±2.1c | 51.6±2.3c | 37.8±0.5c | 15.2±1.4b | 7.1±1.1b | 45.2±3.0 |
(+)-Catechin2) | 99.7±0.1a | 97.5±0.1a | 85.2±1.2a | 39.3±1.1a | 32.7±0.7a | 14.6±0.4 |
Scavenging activities are expressed as the mean±SD of triplicate experiments. Mean separation within columns of respective seasons by Duncan’s multiple range test at p=0.05.
(+)-Catechin was used as a positive control.