| Freezing conditions | -20°C still-air | -40°C still-air | -40°C immersed-liquid |
| Thawing conditions and instrument | - Room temperature (20 C) |
| - Incubator (B.O.D. Incubator VS-1203P1, Vision Scientific, Bucheon, Korea) |
| Freezing storage conditions | at -20°C for 210 days |
| (0, 15, 30, 60, 90, 120, 150, 180, 210 days) |
| Freezing time (min) | 4,410 | 1,920 | 80 |
| Freezer information | Digital ez GC-124HGFP | DSS-650TD080 (Daesan ENG Co., Ltd., Hanam, Korea) | F-500 (Top Greentech, Seoul, Korea) |
| (LG electronics Co., Ltd., Changwon, |
| Korea) |