Table 4 Antioxidant ability of Doenjang fermented with Aspergillus oryzae SCF-6 and protease

Fermentation period (week) F-value
0 2 4 8
DPPH radical scavenging activity (%) Control 8.93±4.93Ab2) 12.04±0.49Bb 22.45±2.90Aa 28.87±3.75Aa 0.0003
Protease A1) 13.20±4.81Ab 18.74±2.74Ab 26.69±2.18Aa 30.45±2.07Aa 0.0006
Protease B 12.51±2.50Ac 23.52±0.49Ab 25.01±2.96Ab 33.98±1.20Aa <0.0001
Protease A+B 11.02±4.24Ac 20.64±4.67Ab 26.22±3.99Aab 33.38±2.82Aa 0.0008
F-value 0.6333 0.005 0.3899 0.2251
Total phenol (mg TAE/g) Control 0.60±0.06Ad 0.92±0.10Bc 1.66±0.05Ab 1.86±0.08Ca <0.0001
Protease A 0.77±0.13Ad 1.11±0.12ABc 1.50±0.06Bb 2.01±0.09Ba <0.0001
Protease B 0.78±0.11Ad 1.26±0.04Ac 1.56±0.00ABb 2.24±0.01Aa <0.0001
Protease A+B 0.70±0.04Ad 1.07±0.20ABc 1.56±0.07ABb 2.20±0.07Aa <0.0001
F-value 0.1344 0.0633 0.0402 0.0005
Protease A isolated from Bacillus licheniformis, Protease B isolated from Aspergillus species, Protease A+B=0.5:0.5 ratio.
Any means in the same fermentation time (A-B) or enzyme (a-d) followed by different letters are significantly (p<0.05) different by Duncan’s multiple range test.