Table 4 Chromosomal aberration test on gamma-irradiated balanced nutritional formular at 4 kGy using a lung cell line of Chinese hamster

Test compound	Presence of S9	Conc. (μg/mL)	G1)	CD	CX	TD	TX	Others	Nor	Total
Hot water extract	+	0	0	0	0	0	0	0	100	100
	+	625	1	0	0	0	0	0	99	100
	+	1,250	1	0	0	0	0	0	99	100
	+	2,500	1	0	0	0	1	0	98	100
	+	5,000	1	0	1	0	0	0	98	100
Methanol extract	+	0	0	0	0	0	0	0	100	100
	+	625	0	0	0	0	0	0	100	100
	+	1,250	1	0	0	0	0	0	99	100
	+	2,500	0	0	0	0	0	0	100	100
	+	5,000	1	0	0	0	0	0	99	100
BP2)	+	20,000	2	0	3	4	14	0	77	100
Hot water extract	-	0	0	0	0	0	0	0	100	100
	-	625	2	0	0	0	0	0	98	100
	-	1,250	0	0	0	0	0	1	99	100
	-	2,500	0	0	0	0	0	0	100	100
	-	5,000	0	0	0	0	1	0	99	100
Methanol extract	-	0	0	0	0	0	0	0	100	100
	-	625	1	0	1	0	0	0	98	100
	-	1,250	0	0	0	0	0	0	100	100
	-	2,500	0	0	1	0	0	0	99	100
	-	5,000	1	0	0	0	0	0	99	100
MMC3)	-	100	7	0	10	1	7	0	75	100

Number of findings of mean aberrant metaphases among 100 metaphases per culture.
G, gaps (chromatid type+chromosome type); CD, chromosome type deletions; CX, chromosome type exchanges; TD, chromatid type deletions; TX, chromatid type exchanges; Others, metaphases with more than 10 aberrations (including gaps) or with chromosomes fragmentation, Nor, normal.
BP, benzopyrene.
MMC, mitomycin C.