Table 3 Salmonella Typhimurium reversion assay of gamma-irradiated balanced nutritional formular at 4 kGy

Test compound1)	Presence of S9	Conc. (μg/plate)	No. of His+ revertant per plate
			TA 98	TA 100	TA 1535	TA 1537
			Hot water extract	+	0	32±22)	110±13	13±4	13±5
	+	321.5	34±5	126±22	11±2	12±1
	+	625	35±5	116±12	12±4	14±2
	+	1,250	36±3	108±4	9±2	16±3
	+	2,500	30±3	99±5	10±1	15±5
	+	5,000	33±4	109±31	16±2	18±1
Methanol extract	+	0	32±3	125±7	14±2	16±4
	+	321.5	31±4	127±10	11±3	13±2
	+	625	37±3	122±19	13±1	15±4
	+	1,250	33±4	106±13	12±4	13±4
	+	2,500	32±3	114±24	15±3	12±3
	+	5,000	30±3	115±8	13±3	11±2
BP	+	1.0	492±41*3)	-	-	-
2-AA	+	1.0	-4)	1,184±224*	257±44*	372±63*
Hot water extract	-	0	37±2	99±13	15±2	14±3
	-	321.5	40±2	91±8	13±3	13±7
	-	625	38±4	84±11	14±3	15±3
	-	1,250	34±5	97±14	10±2	18±5
	-	2,500	36±4	102±13	11±3	12±6
	-	5,000	35±3	88±8	12±3	11±2
Methanol extract	-	0	38±4	123±14	15±3	18±3
	-	321.5	34±4	133±11	12±3	17±2
	-	625	33±4	110±18	14±4	13±5
	-	1,250	35±5	122±15	16±3	12±5
	-	2,500	36±3	107±7	13±2	14±5
	-	5,000	37±3	108±14	11±4	15±2
SA	-	5	-	1,213±189*	735±43*	-
4NQO	-	2	718±38*	-	-	-
ICR-191	-	2	-	-	-	862±36*

BP, benzopyrene; 2-AA, 2-aminoanthracene; SA, Sodium azide; 4NQO, 4-nitroquinoline N-oxide; ICR-191, acridine mutagen ICR-191 were used as positive controls for the corresponding strains.
Each value represents revertant colonies per plate and is expressed in the mean±SD of three plates.
*Significantly different from the negative control (p<0.05).
Not examined.