| Instrument | HITACHI L-8900 Amino acid analyzer |
| Main column | HITACHI HPLC packed column #2622PF column (4.6×60) ion |
| exchange column | |
| Solution | Wako L-8500 Buffer solution PF-1, 2, 3, 4, RG Ninhydrin |
| coloring solution set | |
| Detector | VIS1 : 570 nm |
| VIS2 : 440 nm | |
| UV detector | |
| Injection | 20 μL |