Table 1 Operating conditions of HPLC for the analysis of chlorogenic acid and neochlorogenic acid

Instrument	HPLC (Waters 2695, Waters, USA)
Column	Atlantis dC18 4.6×150 mm, 5 μm
Mobile phase A	Methanol
Mobile phase B	4% Acetic acid
Flow rate	2.0 mL/min
Gradient(%)
	Time (min)	Profile
		A	B
		0	5	95
	5	5	95
	10	40	60
	35	90	10
	40	5	95
	50	5	95
	Injection volume	10 μL
	Oven temperature	30°C
	Detection	UV 360 nm