Table 1
Experimental data on brown color intensity, total phenolic compounds, ABTS radical scavenging activity and Fe2+ chelating activity of the dr ied Gugija (Lycium chinensis Mill) under different conditions based on central composite design for response sur face analysis
Exp. No.1) | Extraction conditions | Brown color intensity (OD at 420 nm) | Total phenolic compounds (μg/100 g) | ABTS radical scavenging activity (%) | Fe2+ chelating activity (%) |
Ethanol conc. (%) | Extraction time(hr) |
1 | 60 ( 1) | 4 ( 1) | 0.8032) | 1,634 | 6.88 | 29.87 |
2 | 60 ( 1) | 2 (-1) | 0.747 | 1,688 | 7.35 | 29.08 |
3 | 20 (-1) | 4 ( 1) | 0.586 | 2,126 | 12.85 | 48.29 |
4 | 20 (-1) | 2 (-1) | 0.455 | 1,711 | 7.93 | 44.44 |
5 | 40 ( 0) | 3 ( 0) | 0.655 | 2,072 | 8.23 | 44.27 |
6 | 40 ( 0) | 3 ( 0) | 0.659 | 2,033 | 8.17 | 43.70 |
7 | 80 ( 2) | 3 ( 0) | 0.704 | 1,427 | 6.88 | 27.40 |
8 | 0 (-2) | 3 ( 0) | 0.425 | 1,296 | 8.31 | 59.36 |
9 | 40 ( 0) | 5 ( 2) | 0.641 | 2,264 | 11.04 | 42.01 |
10 | 40 ( 0) | 1 (-2) | 0.606 | 2,318 | 7.83 | 41.36 |
The number of experimental conditions by central composite design.
Data were expressed as mean of triplicate determinations.