| Parameter | Condition |
| Column | Ion exchange column (4.6 mm×60 mm) |
| Column temperature | 30~70℃ |
| Mobile phase | Pump 1 : buffer solution |
| Pump 2 : ninhydrin | |
| Flow rate | Pump 1 : 0.35 mL/min |
| Pump 2 : 0.3 mL/in | |
| Injection volume | 20 μL |
| Reaction coil temperature | 135℃ |
| Photometer | Channel 1 : UV 570 nm |
| Channel 2 : UV 440 nm |