Table 4
Antioxidant activities of Makgeolli according to different fermentation temperature and periods

Antioxidant test Treated conc. (μg/mL) Antioxidant activities (%)
20℃ 25℃ 30℃ Positive controls
3 days 5 days 7 days 3 days 5 days 7 days 3 days 5 days 7 days BHA α-Tocopherol
DPPH 1,000 46.39±1.47f1) 48.74±0.57ef 48.39±0.54ef 51.26±0.77de 48.15±0.73ef 47.92±0.47ef 47.92±2.42ef 36.48±1.85gh 38.36±2.75g 91.57±0.09a 91.31±0.09a
500 28.97±0.51jk 33.37±0.37hi 30.79±1.30ij 35.31±0.20gh 29.15±1.42jk 33.43±1.63hi 30.38±0.90ij 21.99±0.41m 27.68±1.15jkl 91.26±0.18a 91.52±0.16a
250 18.24±1.41no 20.18±0.37mn 18.06±0.71no 22.17±0.54m 18.71±3.20mno 19.65±1.73mn 18.12±1.50no 12.38±0.30qrs 25.92±0.77kl 89.79±0.16a 91.20±0.16a
125 18.71±1.22mno 15.31±2.80opq 14.02±1.85pqr 18.36±5.70no 15.13±1.86opq 18.65±2.86mno 17.18±0.71nop 17.24±1.80nop 19.94±3.51mn 77.54±0.82b 56.34±0.72c
62.5 10.26±2.60stu 9.91±1.23stu 8.62±0.27tuv 10.85±2.24rst 9.62±2.10stuv 9.21±1.06stuv 7.33±1.63tuv 6.10±0.57vw 13.90±4.17pqr 53.93±0.87cd 25.45±1.32l
31.25 10.03±1.03stu 7.92±1.67tuv 6.63±1.48uvw 9.79±4.22stuv 9.15±2.90stuv 8.45±1.86tuv 9.27±.5.41stuv 6.51±3.37uvw 3.28±1.47w 30.26±1.29ij 12.83±1.25qrs
ABTS 1,000 86.46±0.35bc 87.44±0.35b 87.77±0.51ab 87.61±0.25ab 87.68±0.59ab 87.67±0.49ab 87.79±0.61ab 86.63±0.74bc 86.30±0.40bc 89.44±0.39a 84.84±0.15cd
500 74.08±0.78g 79.79±0.64f 80.03±0.44f 83.41±0.81de 79.17±0.35f 82.24±0.90e 81.84±0.86e 79.07±0.87f 79.23±0.48f 88.07±0.39ab 81.77±0.39e
250 56.67±0.67n 63.74±0.70m 65.85±0.81kl 69.51±0.51i 64.42±0.86lm 68.76±0.77ij 67.85±1.06ij 68.31±0.95ij 66.92±0.16jk 87.22±0.26b 79.56±0.26f
125 37.80±0.36u 45.02±0.53t 48.73±1.33rs 49.91±0.93qr 47.47±1.41s 51.78±0.59op 53.22±5.22o 50.35±1.48pqr 51.36±0.67pq 82.11±0.26e 75.47±1.11g
62.5 21.98±0.50B 27.61±1.52A 30.28±0.87yz 31.22±1.15xyz 29.75±0.85z 33.76±1.81v 31.81±1.05wxy 32.65±0.34vwx 33.36±0.56vw 79.47±0.15f 72.40±1.35h
31.25 11.87±0.42F 16.20±0.88E 16.78±0.61E 17.19±0.43DE 17.26±0.46DE 19.05±0.41CD 17.90±0.96DE 18.72±0.55CD 20.41±0.32BC 71.29±1.70h 67.29±1.93jk
Antioxidant test Treated conc. (μg/mL) 20℃ 25℃ 30℃ Positive control
3 days 5 days 7 days 3 days 5 days 7 days 3 days 5 days 7 days Trolox
FRAP 1000 34.35±0.46h 38.29±0.98g 41.51±0.59ef 43.64±0.23d 42.33±0.92de 40.62±0.41f 37.55±0.46g 31.60±1.13i 28.81±0.93j 92.27±0.25a
500 18.48±1.19m 23.06±1.31l 24.35±0.32l 28.01±0.39j 23.00±0.79l 26.13±0.00k 23.36±0.25l 18.81±0.28m 16.15±1.11n 92.26±0.07a
250 11.73±0.66qr 13.77±0.72op 14.31±0.46o 16.66±0.67n 12.30±0.32pqr 15.72±0.30n 13.04±0.32opq 12.30±0.32pqr 11.10±2.80r 92.66±0.07a
125 6.39±0.56uv 7.86±0.78t 8.68±0.47st 9.66±1.97s 6.17±0.74uvw 8.36±1.69st 7.24±0.65tu 7.86±0.18t 5.30±1.15vwxy 92.17±0.05a
62.51 1.79±1.24DEF 3.76±0.34zAB 4.65±0.84xyzA 5.74±0.65vwx 4.08±1.21yzAB 4.76±0.33wxyz 3.07±0.34ABCD 3.64±0.20zABC 0.84±1.03ef 90.19±0.20b
31.25 1.43±1.09EF 3.28±1.57zABCD 2.96±0.53BCD 3.41±0.91zABC 2.15±0.53CDE 3.18±0.72zABCD 2.96±0.53BCD 3.64±0.39zABC 0.49±0.21f 81.13±0.10c
Values represent the mean±SD (n=3). Means with different superscript with the column are significantly different (p<0.05) by Duncan’s multiple range test.