Table 2. Minimum inhibitory concentration (mg/mL) of pure isothiocyanates and radish root hydrolysate against fish pathogenic bacter ia

Isothiocyanates		Fish pathogenic bacteria
		S. parauberis	S. iniae	E. tarda	V. ichthyoenteri	V. harveyi	P. damselae
		Aliphatic ITC1)
	Sulforaphane	0.500±0.000bc3)	0.094±0.036ef	0.063±0.000ef	1.000±0.000b	0.125±0.000cd	0.188±0.072bc
	Sulforaphene	0.125±0.000cd	0.250±0.000d	0.078±0.031ef	1.000±0.000b	0.125±0.000cd	0.250±0.000bc
	Iberin	0.156±0.063cd	0.250±0.000d	0.188±0.210de	1.000±0.000b	0.250±0.000c	0.438±0.125b
	Erucin	0.750±0.289b	0.094±0.036ef	0.063±0.000ef	1.000±0.000b	0.063±0.000cd	0.125±0.000c
	Allyl ITC	0.750±0.289b	>4.000	0.500±0.000c	>4.000	0.500±0.354b	0.667±0.289a
	Hexyl ITC	>4.000	4.000±0.000a	>4.000	>4.000	>4.000	>4.000
Aromatic ITC
	Phenylethyl ITC	0.188±0.072cd	0.625±0.250c	0.250±0.000d	0.625±0.250c	0.125±0.000cd	0.219±0.063bc
	Benzyl ITC	0.500±0.000bc	0.219±0.063de	0.313±0.125d	0.750±0.289bc	0.125±0.000cd	0.375±0.144bc
	Phenyl ITC	1.500±0.577a	2.000±0.0000b	4.000±0.000a	4.000±0.000a	4.000±0.000a	>4.000
Indolyl ITC
	Indole-3-carbinol	0.375±0.144bcd	0.125±0.000def	1.000±0.000b	0.313±0.125d	0.500±0.000b	0.375±0.144bc
Hydrolysate of radish root		0.438±0.125bc	0.250±0.000d	0.500±0.000c	>0.500	0.500±0.000b	>0.500
Oxytetracycline2)		0.0003±0.0000d	0.0027±0.0008f	0.0007±0.0000f	0.0009±0.0004e	0.0004±0.0000d	0.350±0.100bc

ITC, isothiocyanate.
Oxytetracycline, positive control.
Values are expressed as mean±SD (n=4). Means with different superscripts in the same column are significantly different by Duncan’s multiple rage test (p<0.05).