Table 2.
Operating conditions of HPLC for analyzing organic acid contents

Items Conditions
Instrument Agilent 1260 Infinity
Column PL Hi-Plex H (ɸ7.7 mm×300 mm, Agilent)
Column temp. 65°C
Detector UV Detector
Mobile phase 0.005 M Sulfuric acid
Flow rate 0.6 mL/min
Injection volume 20 μL
Running time 20 min