Table 4.
Estimation of shelf-life based on the regression equation of viable cell count dur ing storage at 20 and 4°C

Storage temp.(°C)	Regression equation	r2	Estimated shelf-life (week)1)
-20	y = 0.1311x + 3.5545	0.8569	14.9
4	y = 0.7763x + 5.6087	0.5468	0.4

Calculated period was calculated by regression equation at 6 log CFU/g of aerobic plate counts under -20 and 4°C storage. Estimated shelf life=calculated shelf life×safety factor (0.8).